酿酒酵母中线粒体在信使核糖核酸产生过程中的自主性。
Autonomy of mitochondria in Saccharomyces cerevisiae in their production of messenger RNA.
作者信息
Mahler H R, Dawidowicz K
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):111-4. doi: 10.1073/pnas.70.1.111.
Abstract
In ts(-)136, a temperature-sensitive mutant of Saccharomyces cerevisiae, nuclear and mitochondrial RNA production can be inhibited selectively by exposure to 36 degrees and ethidium bromide, respectively. Using the programming of mitochondrial polysomes, as measured by their ability to form nascent polypeptide chains, as an assay for functional messenger RNA, we have determined its response to temperature shifts and ethidium bromide. Only ethidium bromide produced a measurable effect; in contrast the cell-sap system responded exclusively to temperature shifts. We conclude that transcription of mitochondrial DNA is sufficient and that import of messenger RNA transcribed from nuclear chromosomes makes no measurable contribution to intramitochondrial protein synthesis.
摘要
在酿酒酵母的温度敏感突变体ts(-)136中，分别通过暴露于36摄氏度和溴化乙锭，可以选择性地抑制核RNA和线粒体RNA的产生。通过线粒体多核糖体形成新生多肽链的能力来衡量其编程情况，以此作为功能性信使RNA的检测方法，我们确定了它对温度变化和溴化乙锭的反应。只有溴化乙锭产生了可测量的影响；相比之下，细胞液系统仅对温度变化有反应。我们得出结论，线粒体DNA的转录是足够的，并且从核染色体转录的信使RNA的导入对线粒体内蛋白质合成没有可测量的贡献。
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