酿酒酵母中核糖核酸合成的抑制剂：信使核糖核酸的降解速率
Inhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid.
作者信息
Tonnesen T, Friesen J D
出版信息
J Bacteriol. 1973 Sep;115(3):889-96. doi: 10.1128/jb.115.3.889-896.1973.
Abstract
Daunomycin and ethidium bromide, two deoxyribonucleic acid-intercalating drugs, inhibit ribonucleic acid (RNA) and protein synthesis in Saccharomyces cerevisiae. Both agents rapidly curtail uptake of radioactive adenine, whereas the kinetics of radioactive leucine uptake after drug addition are consistent with translation of a pool of exponentially decaying messenger RNA. Messenger RNA half-life determinations from these experiments gave identical results over a range of drug concentrations; this value is 21 +/- 4 min at 30 C. In a temperature-sensitive mutant in which RNA synthesis is curtailed at the nonpermissive temperature, a similar half-life for messenger RNA decay is found both in the absence and in the presence of either drug. This indicates that at the concentrations used in this study, neither daunomycin nor ethidium bromide has an appreciable direct effect on translation and do not increase the lability of messenger RNA.
摘要
柔红霉素和溴化乙锭这两种脱氧核糖核酸嵌入药物，可抑制酿酒酵母中的核糖核酸（RNA）和蛋白质合成。两种药物均能迅速减少放射性腺嘌呤的摄取，而添加药物后放射性亮氨酸摄取的动力学与一组呈指数衰减的信使RNA的翻译情况一致。通过这些实验测定信使RNA的半衰期，在一系列药物浓度范围内得到了相同的结果；在30℃时，该值为21±4分钟。在一个温度敏感型突变体中，RNA合成在非允许温度下受到抑制，无论是否存在这两种药物中的任何一种，信使RNA衰变的半衰期都相似。这表明在本研究中使用的浓度下，柔红霉素和溴化乙锭对翻译均无明显直接影响，也不会增加信使RNA的不稳定性。
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