线粒体突变与能量转导之间的偶联
Coupling between mitochondrial mutation and energy transduction.
作者信息
Mahler H R, Bastos R N
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2241-5. doi: 10.1073/pnas.71.6.2241.
Abstract
Upon incubation with ethidium bromide (Etd Br) isolated mitochondria of Saccharomyces cerevisiae have been shown to be capable of performing five novel reactions: (a) a single scission of their DNA (mtDNA) coincident with (b) the incorporation of Etd Br into the product (mtDNA --> 2 mtDNA'-Etd Br); (c) an energy- (and probably ATP-) requiring degradation of mtDNA'-Etd Br to acid-labile products. These reactions acting in series generate (d) a DNase dependent on both Etd Br and an energy supply with mtDNA'-Etd Br as an obligatory intermediate. Coincident with (d) there occurs (e) an activation of adenosinetriphosphatase. Experiments with specific inhibitors suggest that the enzyme responsible is the mitochondrial adenosinetriphosphatase complex itself, and that it and the ability to carry out reactions (a) through (e) are tightly coupled to the energy-transducing functions of the particle.
摘要
用溴化乙锭（Etd Br）孵育后，已证明酿酒酵母分离出的线粒体能够进行五种新反应：（a）其DNA（mtDNA）的单次断裂，同时（b）Etd Br掺入产物中（mtDNA → 2 mtDNA'-Etd Br）；（c）将mtDNA'-Etd Br降解为酸不稳定产物需要能量（可能是ATP）。这些串联反应产生（d）一种依赖于Etd Br和能量供应的DNase，以mtDNA'-Etd Br作为必需中间体。与（d）同时发生（e）腺苷三磷酸酶的激活。用特异性抑制剂进行的实验表明，相关酶是线粒体腺苷三磷酸酶复合体本身，并且它以及进行反应（a）至（e）的能力与该颗粒的能量转导功能紧密耦合。
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本文引用的文献
1
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Biochem Biophys Res Commun. 1968 Feb 15;30(3):232-9. doi: 10.1016/0006-291x(68)90440-3.
2
The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide.酵母中的小菌落突变。用溴化乙锭诱导小菌落时线粒体脱氧核糖核酸的丢失。
J Mol Biol. 1970 Sep 14;52(2):323-35. doi: 10.1016/0022-2836(70)90033-1.
3
Selective inhibition of the in vivo transcription of mitochondrial DNA by ethidium bromide and by acriflavin.溴化乙锭和吖啶黄素对线粒体DNA体内转录的选择性抑制作用。
Biochem Biophys Res Commun. 1970 Nov 25;41(4):1002-8. doi: 10.1016/0006-291x(70)90184-1.
4
Interaction of ethidium with the mitochondrial membrane: cooperative binding and energy-linked changes.溴化乙锭与线粒体膜的相互作用：协同结合及能量相关变化。
Biochem Biophys Res Commun. 1971 Jul 2;44(1):211-7. doi: 10.1016/s0006-291x(71)80180-8.
5
Toward a metabolic interpretation of genetic recombination of E. coli and its phages.关于大肠杆菌及其噬菌体基因重组的代谢解读
Annu Rev Microbiol. 1971;25:437-64. doi: 10.1146/annurev.mi.25.100171.002253.
6
Biogenetic autonomy of mitochondria.线粒体的生物遗传自主性
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7
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