参与与延伸因子G相互作用的核糖体蛋白的鉴定。
Identity of the ribosomal proteins involved in the interaction with elongation factor G.
作者信息
Highland J H, Bodley J W, Gordon J, Hasenbank R, Stöffler G
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):147-50. doi: 10.1073/pnas.70.1.147.
Abstract
Rabbit antibodies produced against 50 of the 55 individually purified ribosomal proteins of Escherichia coli were tested for their ability to interfere with the formation of the ribosome.EF-G.GDP complex. Only antibodies produced against proteins L7 and L12 inhibited complex formation, and they did so completely. These two proteins were previously shown to be immunologically indistinguishable and necessary for the interaction between ribosomes and EF-G. The present data are consistent with the view that the interaction between ribosomes and EF-G that results in GTP hydrolysis occurs on, and is limited to, proteins L7 and L12 on the surface of the 50S ribosomal subunit.
摘要
针对大肠杆菌55种单独纯化的核糖体蛋白中的50种产生的兔抗体，被检测其干扰核糖体-EF-G·GDP复合物形成的能力。只有针对蛋白L7和L12产生的抗体抑制复合物形成，且完全抑制。此前已表明这两种蛋白在免疫学上无法区分，并且对于核糖体与EF-G之间的相互作用是必需的。目前的数据与以下观点一致：导致GTP水解的核糖体与EF-G之间的相互作用发生在50S核糖体亚基表面的蛋白L7和L12上，且仅限于此。
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Ribosomal proteins. Secondary structure of individual ribosomal proteins of E. coli studied by circular dichroism.核糖体蛋白。通过圆二色性研究大肠杆菌单个核糖体蛋白的二级结构。
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Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins.核糖体蛋白。二十五。大肠杆菌核糖体蛋白的免疫学研究。
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Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods.用免疫化学方法测定的大肠杆菌30S核糖体蛋白的序列差异。
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Protection of ribosomes from thiostrepton inactivation by the binding of G factor and guanosine diphosphate.通过G因子和二磷酸鸟苷的结合保护核糖体免受硫链丝菌素失活。
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Ribosomal proteins. XXVI. The number of specific protein binding sites on 16 s and 23 s RNA of Escherichia coli.核糖体蛋白质。二十六。大肠杆菌16 s和23 s RNA上特异性蛋白质结合位点的数量。
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Purification and characterization of 50S ribosomal proteins of Escherichia coli.大肠杆菌50S核糖体蛋白的纯化与特性分析
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Protein biosynthesis.蛋白质生物合成
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