肽链终止过程中对核糖体蛋白L7和L12的需求。
The requirement for ribosomal proteins L7 and L12 in peptide-chain termination.
作者信息
Brot N, Tate W P, Caskey C T, Weissbach H
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):89-92. doi: 10.1073/pnas.71.1.89.
Abstract
Proteins L7 and L12 from 50S ribosomal subunits of Escherichia coli are required for peptidechain termination. This termination process is inhibited by thiostrepton. Since both thiostrepton-treated ribosomes and those depleted of L7 and L12 have a markedly reduced ability to form release factor.UA[(3)H]A.ribosome complexes, the binding of release factors to the ribosome appears to be the primary site of inhibition.
摘要
大肠杆菌50S核糖体亚基中的L7和L12蛋白是肽链终止所必需的。硫链丝菌素可抑制这一终止过程。由于经硫链丝菌素处理的核糖体以及L7和L12缺失的核糖体形成释放因子-UA[（3）H]A.核糖体复合物的能力均显著降低，释放因子与核糖体的结合似乎是抑制的主要位点。
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