Brandenburg A C, Wilson M R
Immunology. 1973 Jan;24(1):119-27.
IgG immunoglobulin was isolated in large quantities from sows' colostrum. The sows were vaccinated intramammarily with a live, formalinized vaccine. A four-stage batch method with DEAE-A50 Sephadex was used in the isolation procedure. Antibacterial tests, heat labile enterotoxin neutralization tests and pig protection tests were employed to investigate the presence of any protective immunity in colostral IgG when compared with whole colostrum. In antibacterial tests, no bactericidal activity was found in colostral whey or colostral IgG from vaccinated or non-vaccinated sows. There was, however, a significant decrease in the rate of multiplication of organisms in tests containing colostral whey or IgG from vaccinated sows when compared with similar preparations from non-vaccinated sows. In enterotoxin neutralization tests using ligated intestinal segments of pigs, the neutralizing effects of colostral whey and IgG on the enterotoxin preparation were significant, whereas only colostral whey afforded significant protection against the vaccine strain of . In pig protection tests, the mean survival time of two groups of 5-day-old gnotobiotic pigs fed colostral whey or IgG was significantly greater than that of the untreated control group. There was no significant difference between the two treated groups.
从母猪初乳中大量分离出免疫球蛋白G（IgG）。母猪通过乳腺内接种活的、经福尔马林处理的疫苗进行免疫。分离过程采用了使用DEAE - A50葡聚糖凝胶的四阶段分批法。与全初乳相比，采用抗菌试验、热不稳定肠毒素中和试验和仔猪保护试验来研究初乳IgG中是否存在任何保护性免疫。在抗菌试验中，未在接种或未接种疫苗的母猪的初乳乳清或初乳IgG中发现杀菌活性。然而，与未接种疫苗的母猪的类似制剂相比，在含有接种疫苗母猪的初乳乳清或IgG的试验中，微生物的繁殖率有显著降低。在使用猪结扎肠段的肠毒素中和试验中，初乳乳清和IgG对肠毒素制剂的中和作用显著；而只有初乳乳清对疫苗株提供了显著保护。在仔猪保护试验中，两组分别喂食初乳乳清或IgG的5日龄无菌仔猪的平均存活时间显著长于未处理的对照组。两个处理组之间没有显著差异。
