Rutter J M, Jones G W, Brown G T, Burrows M R, Luther P D
Infect Immun. 1976 Mar;13(3):667-76. doi: 10.1128/iai.13.3.667-676.1976.
Piglets suckled by dams that had been vaccinated with K88 antigen were significantly more resistant to deaths caused by neonatal diarrhea after challenge with a large dose of a K88-positive enteropathogenic strain of Escherichia coli than piglets suckled by control dams. The factors most likely to be involved in protection of the piglets were investigated by comparing the antibacterial activities of serum and mammary secretions from the two groups of dams. Vaccination stimulated the production of K88 antibodies, which were associated with anti-adhesive activity directed against the adhesive properties of the K88 antigen, and of O8 antibodies; the latter antibodies were attributed to traces of O8 antigen in the vaccine. Neutralizing activity against heat-labile enterotoxin was present in several dams before vaccination but was not stimulated by bacteriostatic activities were similar in serum and mammary secretions from both groups of dams and appeared to play no significant role in the protective after parturition were atrributed to exposure of the dams to the challenge strain excreted by the piglets. It was concluded that neutralization of the adhesive properties of K88 antigen by K88 antibodies in colostrum and in milk contributed significantly to the protection of piglets from vaccinated dams. However, the contribution of antibacterial activities associated with the greater levels of O8 antibodies in colstrum from the vaccinated group cannot be entirely excluded.
与对照组母畜哺育的仔猪相比，由接种过K88抗原的母畜哺育的仔猪在用大剂量K88阳性致病性大肠杆菌菌株攻毒后，对新生仔猪腹泻导致的死亡具有显著更高的抵抗力。通过比较两组母畜血清和乳腺分泌物的抗菌活性，研究了最有可能参与保护仔猪的因素。接种疫苗刺激了K88抗体的产生，这些抗体与针对K88抗原黏附特性的抗黏附活性以及O8抗体有关；后者的抗体归因于疫苗中的微量O8抗原。在接种疫苗前，几只母畜就存在针对不耐热肠毒素的中和活性，但未受到刺激。两组母畜血清和乳腺分泌物中的抑菌活性相似，且似乎在产后保护中未发挥重要作用，这归因于母畜接触了仔猪排出的攻毒菌株。得出的结论是，初乳和乳汁中K88抗体对K88抗原黏附特性的中和作用对保护接种疫苗母畜哺育的仔猪有显著贡献。然而，也不能完全排除接种组初乳中较高水平的O8抗体相关抗菌活性的贡献。
