大鼠和小鼠肝脏染色质中非组蛋白染色质蛋白模式的年龄相关变化。
Age-related changes of the pattern of non-histone chromatin proteins from rat and mouse liver chromatin.
作者信息
Medvedev Z A, Medvedeva M N, Robson L
出版信息
Gerontology. 1979;25(4):219-27. doi: 10.1159/000212343.
The non-histone chromatin (NHC) proteins which are loosely bound to DNA were extracted from young and old mouse and rat liver chromatin by 0.35 M NaCl and fractionated into three groups: water-soluble, 0.14 M NaCl soluble and 0.35 M NaCl soluble. NHC proteins in each fraction were separated by SDS-disc polyacrylamide gel electrophoresis. Special attempts were made to locate minor high-molecular-weight proteins which develop faint bands. It was found that the aging of liver cells is associated with increase in number and quantities of high-molecular-weight NHC proteins in the water-soluble group. Salt-soluble groups of NHC proteins show fewer changes of pattern.
用0.35M氯化钠从幼年和老年小鼠及大鼠肝脏染色质中提取与DNA松散结合的非组蛋白染色质（NHC）蛋白，并将其分为三组：水溶性、0.14M氯化钠可溶性和0.35M氯化钠可溶性。通过SDS-圆盘聚丙烯酰胺凝胶电泳分离各组分中的NHC蛋白。特别尝试定位产生淡带的少量高分子量蛋白。结果发现，肝细胞衰老与水溶性组中高分子量NHC蛋白的数量和含量增加有关。NHC蛋白的盐溶性组显示出较少的模式变化。
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