大鼠肝脏和脾脏染色质中F1组蛋白亚组分模式的年龄变化。

Age changes of the pattern of F1 histone subfractions in rat liver and spleen chromatin.

作者信息

Medvedev Z A, Medvedeva M N, Huschtscha L I

出版信息

Gerontology. 1977;23(5):334-41. doi: 10.1159/000212205.

Abstract

The polyacrylamide gel electrophoresis of total histone extracted from young (3 months) and old (26-27 months) rat tissues does not show age-related differences in the pattern of the main five histones. However, when the lysine-rich F1 histone was extracted from chromatin separately by perchloric acid and purified by Biogel P-60 column chromatography from chromatin of rat liver and spleen, the polyacrylamide gel electrophoresis did show obvious age changes in the distribution of F1, F1o, and F1(00) subfractions. In liver chromatin the increase of rat-specific methionine-containing subfraction (F1met.) was also found.

摘要

从年轻（3个月）和年老（26 - 27个月）大鼠组织中提取的总组蛋白进行聚丙烯酰胺凝胶电泳，结果显示主要的五种组蛋白模式不存在与年龄相关的差异。然而，当通过高氯酸从染色质中单独提取富含赖氨酸的F1组蛋白，并通过Biogel P - 60柱色谱法从大鼠肝脏和脾脏的染色质中进行纯化后，聚丙烯酰胺凝胶电泳确实显示F1、F1o和F1(00)亚组分的分布存在明显的年龄变化。在肝脏染色质中还发现了大鼠特异性含甲硫氨酸亚组分（F1met.）的增加。