稳定的F质粒的质粒相关功能。
Plasmid-associated functions of a stable Flac.
作者信息
Macrina F L, Balbinder E
出版信息
J Bacteriol. 1973 Jan;113(1):183-91. doi: 10.1128/jb.113.1.183-191.1973.
Several functions associated with the stable plasmid, FlacS, have been examined. Our results indicate that the sex pili synthesized by Escherichia coli strains carrying FlacS are altered in some manner as evidenced by a very inefficient adsorption of male-specific phages. On the other hand, FlacS-mediated entry exclusion of related plasmids and plasmid incompatibility function(s) appear normal. The presence of covalently closed circular deoxyribonucleic acid in E. coli strains harboring FlacS indicates that it is an autonomously replicating plasmid. Based on beta-galactosidase levels and the percentage of covalently closed circular deoxyribonucleic acid, it appears that the stability of the FlacS is not the result of multiple copies of this plasmid. FlacS appears larger than its precursor, F(ts114)lac, in sedimentation through alkaline sucrose gradients.
已对与稳定质粒FlacS相关的几种功能进行了研究。我们的结果表明，携带FlacS的大肠杆菌菌株合成的性菌毛以某种方式发生了改变，这一点可通过雄性特异性噬菌体的吸附效率极低得到证明。另一方面，FlacS介导的相关质粒的进入排斥和质粒不相容性功能似乎正常。携带FlacS的大肠杆菌菌株中存在共价闭合环状脱氧核糖核酸，这表明它是一种自主复制的质粒。根据β-半乳糖苷酶水平和共价闭合环状脱氧核糖核酸的百分比，FlacS的稳定性似乎不是该质粒多拷贝的结果。在通过碱性蔗糖梯度沉降时，FlacS看起来比其前体F(ts114)lac更大。
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