Tacker J R, Farhi F, Bulmer G S
Infect Immun. 1972 Aug;6(2):162-7. doi: 10.1128/iai.6.2.162-167.1972.
Human peripheral leukocytes were found to engulf and kill cells of Cryptococcus neoformans. Fewer encapsulated than nonencapsulated cells met this fate, since cryptococcal capsular polysaccharide inhibited phagocytosis. During 10 to 12 hr of incubation of nonencapsulated cells in human serum, sufficient polysaccharide was produced to inhibit phagocytosis by 50%. The polysaccharide inhibitor was found in the sera of four patients with cryptococcosis, but not on the surfaces of their leukocytes. Additional experiments indicated that serum is not essential for effective phagocytosis. However, normal human serum contains anticryptococcal activity which is not inhibited by capsular material. Preliminary findings indicate that the phagocytic index of a patient with cryptococcosis may be correlated with the severity of his disease.
研究发现人类外周血白细胞能够吞噬并杀死新型隐球菌细胞。被吞噬杀死的荚膜型细胞比非荚膜型细胞少，因为隐球菌荚膜多糖会抑制吞噬作用。在人血清中对非荚膜型细胞进行10至12小时的孵育后，会产生足够的多糖，使吞噬作用受到50%的抑制。在四名隐球菌病患者的血清中发现了多糖抑制剂，但在他们白细胞的表面未发现。进一步的实验表明，血清并非有效吞噬作用所必需。然而，正常人血清含有抗隐球菌活性，且不受荚膜物质的抑制。初步研究结果表明，隐球菌病患者的吞噬指数可能与其疾病严重程度相关。
