放射细菌溶解法：一种使用铬-51检测抗霍乱弧菌抗体的新技术。
Radiobacteriolysis: a new technique using chromium-51 for assaying anti-Vibrio cholerae antibodies.
作者信息
Blachman U, Clark W R, Pickett M J
出版信息
Infect Immun. 1973 Jan;7(1):53-61. doi: 10.1128/iai.7.1.53-61.1973.
Abstract
A new method for detecting and quantitating antibodies against Vibrio cholerae is described. The reaction involves the release of radiochromium from prelabeled vibrios in the presence of specific antibody and complement. The entire assay can be completed within 5 hr. The method is highly reproducible, immunologically specific, temperature- and complement-dependent, and significantly more sensitive than other methods currently used for titration of anti-Vibrio cholerae antibodies. The technique is also potentially applicable to titration of antibodies against other gram-negative bacteria.
摘要
本文描述了一种检测和定量抗霍乱弧菌抗体的新方法。该反应涉及在特异性抗体和补体存在的情况下，从预先标记的弧菌中释放放射性铬。整个检测可在5小时内完成。该方法具有高度可重复性、免疫特异性、温度和补体依赖性，并且比目前用于滴定抗霍乱弧菌抗体的其他方法灵敏得多。该技术还可能适用于滴定抗其他革兰氏阴性菌的抗体。
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