Glynn A A, Milne C M
Immunology. 1967 Jun;12(6):639-53.
The killing and lysis of by human serum have been measured simultaneously at frequent intervals for periods of 30–60 minutes. The kinetic effects of varying the amounts of lysozyme, antibacterial antibody and complement have been studied. The rate of lysis is largely controlled by the lysozyme concentration but complement is also necessary. Killing is closely related to complement concentration. Antibody is needed in such small amounts that it is rarely a limiting factor. Inhibition of serum lysozyme by anti-human lysozyme prevents lysis and reduces killing but both are restored to normal by addition of egg-white lysozyme. Both lysis and killing are stopped by bentonite absorption of serum and complete return to normal is not attained by subsequent addition of egg-white lysozyme. In a lysozyme-free system lysis does occur after some delay probably due to the action of complement and antibody alone. In the presence of adequate complement and antibody the loss of complement (measured haemolytically) in bentonite treated serum is inadequate to account for the fall in bactericidal activity. A new bentonite absorbable factor (BAF) essential for complete serum bactericidal power is postulated.
在30 - 60分钟的时间段内，每隔一段时间就同时测量人血清对[细菌名称未给出]的杀灭和裂解情况。研究了改变溶菌酶、抗菌抗体和补体含量的动力学效应。裂解速率在很大程度上受溶菌酶浓度控制，但补体也是必需的。杀灭作用与补体浓度密切相关。所需抗体量极少，因此很少成为限制因素。抗人溶菌酶对血清溶菌酶的抑制会阻止裂解并降低杀灭作用，但通过添加蛋清溶菌酶，两者均可恢复正常。血清经膨润土吸附后，裂解和杀灭作用均停止，随后添加蛋清溶菌酶也无法完全恢复正常。在无溶菌酶的系统中，经过一段时间延迟后确实会发生裂解，这可能仅归因于补体和抗体的作用。在存在足够补体和抗体的情况下，经膨润土处理的血清中补体的损失（通过溶血法测量）不足以解释杀菌活性的下降。推测存在一种对血清完全杀菌能力至关重要的新型膨润土可吸附因子（BAF）。
