Kruyssen F J, de Boer W R, Wouters J T
J Bacteriol. 1980 Oct;144(1):238-46. doi: 10.1128/jb.144.1.238-246.1980.
A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespective of whether the carbon source was glucose, glycerol, galactose, or malate, teichoic acid was the main anionic wall polymer whenever phosphorus was present in excess of the growth requirement, and teichuronic acid predominated in the walls of phosphate-limited cells. The effect of growth rate was studied by varying the dilution rate. However, only under phosphate limitation did the wall composition change with the growth rate: walls prepared from cells grown at dilution rates above 0.5 h-1 contained teichoic as well as teichuronic acid, despite the culture still being phosphate limited. The wall content of the cells did not vary with the nature of the growth limitation, but a correlation was observed between the growth rate and wall content. No indications were obtained that the composition of the peptidoglycan of B. subtilis subsp. niger WM was phenotypically variable.
开展了一项研究，以确定除磷的可利用性之外的其他因素是否参与了枯草芽孢杆菌黑色亚种WM中磷壁酸和磷壁醛酸合成的调控。首先，在稀释率维持在约0.3 h-1的同时改变碳源的性质。无论碳源是葡萄糖、甘油、半乳糖还是苹果酸，只要磷的存在量超过生长需求，磷壁酸就是主要的阴离子细胞壁聚合物，而在磷受限细胞的壁中磷壁醛酸占主导。通过改变稀释率研究生长速率的影响。然而，只有在磷限制条件下，细胞壁组成才随生长速率变化：尽管培养物仍处于磷限制状态，但从稀释率高于0.5 h-1的细胞制备的细胞壁中同时含有磷壁酸和磷壁醛酸。细胞的细胞壁含量不随生长限制的性质而变化，但观察到生长速率与细胞壁含量之间存在相关性。没有迹象表明枯草芽孢杆菌黑色亚种WM的肽聚糖组成存在表型变异。
