开环R因子脱氧核糖核酸中的链特异性切口：线性链与蛋白质细胞成分的连接。
Strand-specific nick in open circular R-factor deoxyribonucleic acid: attachment of the linear strand to a proteinaceous cellular component.
作者信息
Morris C F, Hershberger C L, Rownd R
出版信息
J Bacteriol. 1973 Apr;114(1):300-8. doi: 10.1128/jb.114.1.300-308.1973.
Abstract
The fraction of covalently closed circular R-factor R12 deoxyribonucleic acid (DNA) isolated from Proteus mirabilis was found to depend upon the pH of the buffer used to suspend the cells at the time of lysis. Examination of the open circular DNA revealed that there is one strand-specific nick per R-factor molecule. This same DNA strand also appeared to be bound to a proteinaceous particulate fraction of the cell.
摘要
从奇异变形杆菌中分离出的共价闭合环状R因子R12脱氧核糖核酸（DNA）的比例，被发现取决于裂解时用于悬浮细胞的缓冲液的pH值。对开环DNA的检查表明，每个R因子分子有一个链特异性切口。同一条DNA链似乎也与细胞的蛋白质颗粒部分结合。
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