一类新型抗肿瘤药物。II. 体外试验。

A novel class of antitumour agents. II. In vitro testing.

作者信息

Emminger A, Kahmann E, Savage D S

机构信息

Abteilung für Experimentelle Pathologie, Medizinische Hochschule Hannover, G.F.R.

出版信息

Cancer Lett. 1977 Mar;2(4-5):273-8. doi: 10.1016/s0304-3835(77)80032-3.

DOI:10.1016/s0304-3835(77)80032-3

PMID:45730

Abstract

Suspensions of Walker and HeLa cells, rat fibroblasts and human stimulated lymphocytes were incubated over various periods with the synthetic amino steroid, 2 beta,16 beta-dipiperidino-5 alpha-androstane-3 alpha,17 beta-diol dipivalate (DAP). The acute cell destroying effect was found to be exponentially time and dose dependent within a certain range. With lower nontoxic doses, decreases in the mitotic and labelling indices were observed, and clotted mitotic figures occurred. The effects were essentially the same for all experimental cell types; chromosome alterations were not seen.

摘要

将沃克细胞和海拉细胞、大鼠成纤维细胞以及人刺激淋巴细胞的悬液与合成氨基类固醇2β,16β - 二哌啶基 - 5α - 雄甾烷 - 3α,17β - 二醇二特戊酸酯（DAP）在不同时间段进行孵育。发现在一定范围内，急性细胞破坏效应呈指数时间和剂量依赖性。使用较低的无毒剂量时，观察到有丝分裂指数和标记指数降低，并且出现了凝缩的有丝分裂图像。所有实验细胞类型的效应基本相同；未观察到染色体改变。