Dawson K A, Preziosi M C, Caldwell D R
J Bacteriol. 1979 Aug;139(2):384-92. doi: 10.1128/jb.139.2.384-392.1979.
Uncouplers and inhibitors of electron transport affected growth and electron transport of rumen bacteria in various ways. Selenomonas ruminantium was not affected by inhibitor and uncoupler concentrations which affected growth and electron transport of Bacteroides ruminicola, B. succinogenes, and Butyrivibrio fibrisolvens. Inhibitors, when active, led to accumulation of reduced electron carriers before the site of action, but differences were found among organisms in the site of action of these inhibitors. Uncouplers reduced the glucose molar growth yields (Ygluc) of B. ruminicola, B. succinogenes, and B. fibrisolvens compared with those obtained without uncouplers. The extent of Ygluc reduction accompanying inhibitor exposure reflected electron transport chain structure. S. ruminantium appeared to obtain its adenosine 5'-triphosphate from substrate-level processes only. The other organisms studied appeared to obtain adenosine 5'-triphosphate both from substrate-level processes and from electron transport but differed in the amount of adenosine 5'-triphosphate obtained from glucose catabolism and in the proportions of adenosine 5'-triphosphate obtained from substrate-level reactions and electron transport.
解偶联剂和电子传递抑制剂以多种方式影响瘤胃细菌的生长和电子传递。反刍月形单胞菌不受影响瘤胃栖粪杆菌、琥珀酸栖粪杆菌和溶纤维丁酸弧菌生长及电子传递的抑制剂和解偶联剂浓度的影响。抑制剂发挥作用时，会导致在作用位点之前还原态电子载体的积累，但在这些抑制剂的作用位点上，不同微生物之间存在差异。与未添加解偶联剂时相比，解偶联剂降低了瘤胃栖粪杆菌、琥珀酸栖粪杆菌和溶纤维丁酸弧菌的葡萄糖摩尔生长产率（Ygluc）。伴随抑制剂暴露的Ygluc降低程度反映了电子传递链结构。反刍月形单胞菌似乎仅从底物水平过程中获取三磷酸腺苷。所研究的其他微生物似乎既从底物水平过程又从电子传递中获取三磷酸腺苷，但在从葡萄糖分解代谢中获取的三磷酸腺苷量以及从底物水平反应和电子传递中获取的三磷酸腺苷比例方面存在差异。
