核糖体核糖核酸顺反子在酵母染色体中的分布。
Distribution of ribosomal ribonucleic acid cistrons among yeast chromosomes.
作者信息
de Kloet S R
出版信息
J Bacteriol. 1973 Jun;114(3):1034-9. doi: 10.1128/jb.114.3.1034-1039.1973.
High-molecular-weight deoxyribonucleic acid (DNA) of Saccharomyces carls bergensis has been fractionated by sucrose density gradient centrifugation. The main DNA fraction has an average molecular weight of about 500 x 10(6). A major fraction of the DNA molecules containing sequences homologous to ribosomal ribonucleic acid (RNA) sediments as material of this molecular weight. The remainder sediments as material of a molecular weight of about 250 x 10(6). The latter fraction contains relatively more ribosomal RNA cistrons than the former. Studies on the buoyant density of high-molecular-weight DNA homologous to ribosomal RNA have led to the conclusion that the ribosomal RNA cistrons occur in groups attached to a relatively large amount of nonribosomal RNA and suggest that ribosomal RNA cistrons are distributed over a number of yeast chromosomes.
卡尔酵母的高分子量脱氧核糖核酸（DNA）已通过蔗糖密度梯度离心法进行了分级分离。主要的DNA级分平均分子量约为500×10⁶。含有与核糖体核糖核酸（RNA）同源序列的大部分DNA分子以该分子量的物质形式沉降。其余部分以分子量约为250×10⁶的物质形式沉降。后一级分比前一级分含有相对更多的核糖体RNA顺反子。对与核糖体RNA同源的高分子量DNA的浮力密度研究得出结论，核糖体RNA顺反子成组存在，附着于相对大量的非核糖体RNA上，并表明核糖体RNA顺反子分布在多个酵母染色体上。
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