Yabusaki K K, Cohen R E, Ballou C E
J Biol Chem. 1979 Aug 10;254(15):7282-6.
The anomeric proton magnetic resonances of Mycobacterium smegmatis 3-O-methylmannose polysaccharide have chemical shifts intermediate betwen those of nonaglucoamylose and alpha-cyclodextrin, and on addition of palmitic acid most of these resonances are shifted upfield toward that of the cyclodextrin. This suggests that the methylated polysaccharide could have a conformation with some secondary structure intermediate between those of the two reference compounds, and that it forms a tightened coil upon addition of the lipid which yields an inclusion complex with the polysaccharide. The change in chemical shift is linear with lipid concentration, which indicates that the complex undergoes rapid exchange with free polysaccharide. The changes in proton chemical shifts of the polysaccharide and of the palmitic acid are consistent with the fatty acid being inserted in the coiled polysaccharide with its carboxyl group near the methyl aglycon.
耻垢分枝杆菌3 - O - 甲基甘露糖多糖的端基质子磁共振化学位移介于非阿葡糖直链淀粉和α - 环糊精之间，加入棕榈酸后，这些共振信号大多向环糊精的方向上移。这表明甲基化多糖可能具有一种构象，其二级结构介于两种参考化合物之间，并且在加入脂质后会形成紧密的螺旋结构，与多糖形成包合物。化学位移的变化与脂质浓度呈线性关系，这表明该复合物与游离多糖之间进行快速交换。多糖和棕榈酸的质子化学位移变化与脂肪酸以其羧基靠近甲基苷元的方式插入卷曲的多糖中相一致。
