Frangione B, Lee L, Haber E, Bloch K J
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1073-7. doi: 10.1073/pnas.70.4.1073.
Protein Hal is a human gamma4 heavy-chain disease protein whose molecular weight is reduced from 55,000 to 25,000 in 6 M guanidine due to the lack of disulfide bonds between heavy chains. Studies of aminoacid sequence indicate that it contains a gap of about 240 residues, starting 10 residues from the N-terminal end and including the rest of the Fd fragment, as well as the hinge region. Normal sequence apparently resumes at a methionine residue (position 252) in the second constant domain (C(H)2) and seems normal from there to the carboxyl end of the molecule. These results imply that reinitiation of translation at an internal AUG codon occurs in protein Hal.
蛋白质Hal是一种人类γ4重链病蛋白，在6M胍中，由于重链之间缺乏二硫键，其分子量从55,000降至25,000。氨基酸序列研究表明，它包含一个约240个残基的缺口，从N末端起始10个残基处开始，包括Fd片段的其余部分以及铰链区。正常序列显然在第二个恒定结构域（C(H)2）中的一个甲硫氨酸残基（第252位）处重新开始，并且从那里到分子的羧基末端似乎是正常的。这些结果表明，蛋白质Hal中发生了内部AUG密码子处的翻译重新起始。
