色氨酸阻遏物对色氨酸操纵子转录的体外抑制作用。
In vitro repression of transcription of the tryptophan operon by trp repressor.
作者信息
Shimizu Y, Shimizu N, Hayashi M
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1990-4. doi: 10.1073/pnas.70.7.1990.
Abstract
The in vitro repression of transcription of the tryptophan operon by the trp repressor from Escherichia coli was studied. By measuring the inhibitory effect for trp-specific RNA synthesis in an in vitro transcription system directed by DNA of trp-transducing phage, we have detected and concentrated a trp repressor in an eluate of a Phi80 ptED native DNA-cellulose column. The repression of transcription of trp operon required the addition of L-tryptophan in the system, and when several tryptophan analogues were added, the repression or derepression was similar to that observed in vivo. The repressor fraction was separated from the majority of tryptophanyl-tRNA synthetase activity by Bio-gel P60 column chromatography.
摘要
对来自大肠杆菌的色氨酸阻遏物在体外对色氨酸操纵子转录的抑制作用进行了研究。通过在由色氨酸转导噬菌体DNA指导的体外转录系统中测量对色氨酸特异性RNA合成的抑制作用，我们在Phi80 ptED天然DNA-纤维素柱的洗脱液中检测并浓缩了一种色氨酸阻遏物。色氨酸操纵子转录的抑制需要在系统中添加L-色氨酸，当添加几种色氨酸类似物时，抑制或去抑制情况与体内观察到的相似。通过Bio-gel P60柱色谱法将阻遏物组分与大部分色氨酰-tRNA合成酶活性分离。
相似文献
1
In vitro repression of transcription of the tryptophan operon by trp repressor.色氨酸阻遏物对色氨酸操纵子转录的体外抑制作用。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1990-4. doi: 10.1073/pnas.70.7.1990.
2
Tryptophanyl-tRNA and tryptophanyl-tRNA synthetase are not required for in vitro repression of the tryptophan operon.色氨酸-tRNA和色氨酸-tRNA合成酶对于色氨酸操纵子的体外阻遏不是必需的。
Nat New Biol. 1973 Oct 3;245(144):131-3. doi: 10.1038/newbio245131a0.
3
Synthesis of tryptophan operon RNA in a cell-free system.
Nat New Biol. 1973 Oct 3;245(144):137-40. doi: 10.1038/newbio245137a0.
4
Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan.在部分纯化的阻遏物和色氨酸存在的情况下，利用纯化的RNA聚合酶对色氨酸操纵子进行体外转录调控。
Nat New Biol. 1973 Oct 3;245(144):133-7. doi: 10.1038/newbio245133a0.
5
Interaction of the operator of the tryptophan operon with repressor.色氨酸操纵子的操纵基因与阻遏物的相互作用。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3134-8. doi: 10.1073/pnas.71.8.3134.
6
Dual-control of the tryptophan operon is mediated by both tryptophanyl-tRNA synthetase and the repressor.色氨酸操纵子的双重调控由色氨酰 - tRNA合成酶和阻遏物共同介导。
J Mol Biol. 1976 May 15;103(2):209-26. doi: 10.1016/0022-2836(76)90310-7.
7
Transcription of the tryptophan operon of Escherichia coli in vitro. I. Detection and quantitative determination of specific RNA.大肠杆菌色氨酸操纵子的体外转录。I. 特异性RNA的检测与定量测定
Biochim Biophys Acta. 1968 Dec 17;169(2):494-510. doi: 10.1016/0005-2787(68)90058-0.
8
Repression of tryptophan operon RNA synthesis by trp repressor in an in vitro coupled transcription-translation system.在体外偶联转录-翻译系统中，色氨酸阻遏物对色氨酸操纵子RNA合成的抑制作用。
FEBS Lett. 1974 Mar 15;40(1):80-3. doi: 10.1016/0014-5793(74)80898-7.
9
Isolation and characterization of specialized phi80 transducing phages carrying regions of the Salmonella typhimurium trp operon.携带鼠伤寒沙门氏菌色氨酸操纵子区域的特异性φ80转导噬菌体的分离与鉴定。
J Bacteriol. 1974 May;118(2):505-13. doi: 10.1128/jb.118.2.505-513.1974.
10
Initiation sites for in vitro transcription of the tryptophan operon.色氨酸操纵子的体外转录起始位点。
Biochemistry. 1974 Dec 3;13(25):5235-42. doi: 10.1021/bi00722a029.
引用本文的文献
1
Bioinformatic prediction reveals posttranscriptional regulation of the chromosomal replication initiator gene by the attenuator sRNA rnTrpL in .生物信息学预测表明，在 中，衰减子 sRNA rnTrpL 对染色体复制起始基因进行转录后调控。
RNA Biol. 2021 Sep;18(9):1324-1338. doi: 10.1080/15476286.2020.1846388. Epub 2020 Nov 19.
2
A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.一种影响色氨酸阻遏蛋白与含操纵基因的DNA之间结合模式的大肠杆菌固定相蛋白。
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5796-800. doi: 10.1073/pnas.90.12.5796.
3
DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.大肠杆菌色氨酸阻遏蛋白基因（trpR基因）的DNA序列及色氨酸阻遏蛋白氨基酸序列预测
Nucleic Acids Res. 1980 Apr 11;8(7):1551-60. doi: 10.1093/nar/8.7.1551.
4
Analysis in vivo of factors affecting the control of transcription initiation at promoters containing target sites for trp repressor.对影响含有色氨酸阻遏物靶位点的启动子转录起始控制的因素进行体内分析。
Mol Gen Genet. 1984;193(1):110-8. doi: 10.1007/BF00327423.
5
Interaction of the operator of the tryptophan operon with repressor.色氨酸操纵子的操纵基因与阻遏物的相互作用。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3134-8. doi: 10.1073/pnas.71.8.3134.
6
Molecular dynamics studies of a DNA-binding protein: 2. An evaluation of implicit and explicit solvent models for the molecular dynamics simulation of the Escherichia coli trp repressor.一种DNA结合蛋白的分子动力学研究：2. 对大肠杆菌色氨酸阻遏物分子动力学模拟中隐式和显式溶剂模型的评估
Protein Sci. 1992 Sep;1(9):1185-205. doi: 10.1002/pro.5560010912.
7
Regulation of enzyme synthesis in the tryptophan pathway of Acinetobacter calcoaceticus.醋酸钙不动杆菌色氨酸途径中酶合成的调控。
J Bacteriol. 1976 Jul;127(1):367-79. doi: 10.1128/jb.127.1.367-379.1976.
8
Gene rearrangements in the evolution of the tryptophan synthetic pathway.色氨酸合成途径进化中的基因重排。
Bacteriol Rev. 1975 Jun;39(2):87-120. doi: 10.1128/br.39.2.87-120.1975.
9
Role of ribonucleic acid polymerase in gene selection in procaryotes.核糖核酸聚合酶在原核生物基因选择中的作用。
Bacteriol Rev. 1977 Sep;41(3):568-94. doi: 10.1128/br.41.3.568-594.1977.
10
Transcription of the argF and argI genes of the arginine biosynthetic regulon of Escherichia coli K12, performed in vitro.在体外对大肠杆菌K12精氨酸生物合成调节子的argF和argI基因进行转录。
Mol Gen Genet. 1977 Sep 21;155(1):7-18. doi: 10.1007/BF00268555.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
ON THE TRANSCRIPTION OF THE TRYTOPHAN OPERON IN ESCHERICHIA COLI. I. THE TRYPTOPHAN OPERATOR.关于大肠杆菌中色氨酸操纵子的转录。I. 色氨酸操纵基因
J Mol Biol. 1965 Jan;11:54-63. doi: 10.1016/s0022-2836(65)80170-x.
3
A CHEMICAL BASIS FOR THE HOST-INDUCED MODIFICATION OF T-EVEN BACTERIOPHAGES.宿主诱导T偶数噬菌体修饰的化学基础
Proc Natl Acad Sci U S A. 1963 Aug;50(2):300-5. doi: 10.1073/pnas.50.2.300.
4
Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli.大肠杆菌中控制色氨酸合成的基因间连锁关系的转导与重组研究。
Virology. 1959 Aug;8:425-47. doi: 10.1016/0042-6822(59)90046-7.
5
[Inhibition of the synthesis of the enzymes participating in the formation of tryptophan in Escherichia coli].[对大肠杆菌中参与色氨酸形成的酶的合成的抑制作用]
C R Hebd Seances Acad Sci. 1959 Jun 15;248(24):3490-2.
6
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
7
The tryptophan operon regulated by phage immunity.由噬菌体免疫调节的色氨酸操纵子。
J Mol Biol. 1965 Dec;14(2):608-10. doi: 10.1016/s0022-2836(65)80212-1.
8
Evidence that tryptophanyl transfer ribonucleic acid is not the corepressor of the tryptophan operon of Escherichia coli.色氨酰转移核糖核酸不是大肠杆菌色氨酸操纵子的辅阻遏物的证据。
J Bacteriol. 1971 Jan;105(1):268-75. doi: 10.1128/jb.105.1.268-275.1971.
9
Regulation of the enzymes of the tryptophan pathway in Escherichia coli.大肠杆菌中色氨酸途径中酶的调控
Genetics. 1965 Dec;52(6):1303-16. doi: 10.1093/genetics/52.6.1303.
10
On the transcription of the tryptophan operon in Escherichia coli. 3. Multicistronic messenger RNA and polarity for transcription.关于大肠杆菌中色氨酸操纵子的转录。3. 多顺反子信使核糖核酸与转录极性
J Mol Biol. 1965 Aug;13(1):169-82. doi: 10.1016/s0022-2836(65)80087-0.
Zotero 插件