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大肠杆菌色氨酸阻遏蛋白基因（trpR基因）的DNA序列及色氨酸阻遏蛋白氨基酸序列预测

DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.

作者信息

Singleton C K, Roeder W D, Bogosian G, Somerville R L, Weith H L

出版信息

Nucleic Acids Res. 1980 Apr 11;8(7):1551-60. doi: 10.1093/nar/8.7.1551.

DOI:10.1093/nar/8.7.1551
PMID:7001368
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324016/
Abstract

A DNA sequence of 1041 base pairs from a BamHI fragment containing the E. coli trpR gene has been determined. With this sequence and other experimental evidence, the primary structure (88 amino acids) of the Trp repressor can be predicted. Additional features of the DNA sequences include a 22 base pair region upstream from the proposed structural gene which exhibits striking homology with the trp operator, thus implying that expression of the trpR gene may be under autogenous regulation.

摘要

已确定来自含有大肠杆菌色氨酸阻遏蛋白基因（trpR基因）的BamHI片段的一段1041个碱基对的DNA序列。根据这一序列及其他实验证据，可以预测色氨酸阻遏蛋白的一级结构（88个氨基酸）。该DNA序列的其他特征包括，在假定的结构基因上游有一个22个碱基对的区域，它与色氨酸操纵基因表现出显著的同源性，因此意味着trpR基因的表达可能受自身调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/324016/28f49064596c/nar00424-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/324016/28f49064596c/nar00424-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/324016/28f49064596c/nar00424-0115-a.jpg

相似文献

1
DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.大肠杆菌色氨酸阻遏蛋白基因（trpR基因）的DNA序列及色氨酸阻遏蛋白氨基酸序列预测
Nucleic Acids Res. 1980 Apr 11;8(7):1551-60. doi: 10.1093/nar/8.7.1551.
2
Trp aporepressor production is controlled by autogenous regulation and inefficient translation.色氨酸阻遏物的产生受自身调节和低效翻译的控制。
Proc Natl Acad Sci U S A. 1982 May;79(10):3120-4. doi: 10.1073/pnas.79.10.3120.
3
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.大肠杆菌色氨酸脱辅基阻遏物的结构基因trpR的核苷酸序列及表达
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21. doi: 10.1073/pnas.77.12.7117.
4
The tryptophan repressor sequence is highly conserved among the Enterobacteriaceae.色氨酸阻遏物序列在肠杆菌科细菌中高度保守。
Nucleic Acids Res. 1994 May 25;22(10):1821-9. doi: 10.1093/nar/22.10.1821.
5
Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.含大肠杆菌染色体trpR-thr区域的克隆脱氧核糖核酸的结构与功能分析。
J Bacteriol. 1979 Oct;140(1):106-13. doi: 10.1128/jb.140.1.106-113.1979.
6
Frameshifting in the expression of the E. coli trpR gene occurs by the bypassing of a segment of its coding sequence.大肠杆菌trpR基因表达中的移码现象是通过跳过其编码序列的一段来发生的。
Cell. 1993 Jan 15;72(1):121-30. doi: 10.1016/0092-8674(93)90056-v.
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Frameshifting in the expression of the Escherichia coli trpR gene.大肠杆菌trpR基因表达中的移码现象。
Mol Microbiol. 1992 Oct;6(19):2777-84. doi: 10.1111/j.1365-2958.1992.tb01457.x.
8
Analysis in vivo of factors affecting the control of transcription initiation at promoters containing target sites for trp repressor.对影响含有色氨酸阻遏物靶位点的启动子转录起始控制的因素进行体内分析。
Mol Gen Genet. 1984;193(1):110-8. doi: 10.1007/BF00327423.
9
A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.一种影响色氨酸阻遏蛋白与含操纵基因的DNA之间结合模式的大肠杆菌固定相蛋白。
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5796-800. doi: 10.1073/pnas.90.12.5796.
10
Mutational studies with the trp repressor of Escherichia coli support the helix-turn-helix model of repressor recognition of operator DNA.对大肠杆菌色氨酸阻遏物进行的突变研究支持阻遏物识别操纵基因DNA的螺旋-转角-螺旋模型。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):483-7. doi: 10.1073/pnas.82.2.483.

引用本文的文献

1
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
2
The tryptophan repressor sequence is highly conserved among the Enterobacteriaceae.色氨酸阻遏物序列在肠杆菌科细菌中高度保守。
Nucleic Acids Res. 1994 May 25;22(10):1821-9. doi: 10.1093/nar/22.10.1821.
3
Electrostatic forces contribute to interactions between trp repressor dimers.

本文引用的文献

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Regulatory functions of the lambda repressor reside in the amino-terminal domain.lambda 阻遏物的调控功能位于氨基端结构域。
Nature. 1979 May 31;279(5712):396-400. doi: 10.1038/279396a0.
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[Inhibition of the synthesis of the enzymes participating in the formation of tryptophan in Escherichia coli].[对大肠杆菌中参与色氨酸形成的酶的合成的抑制作用]
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Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.大肠杆菌色氨酸脱辅基阻遏物的结构基因trpR的核苷酸序列及表达
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21. doi: 10.1073/pnas.77.12.7117.
9
Evidence that repression mechanisms can exert control over the thr, leu, and ilv operons of Escherichia coli K-12.有证据表明，阻遏机制可对大肠杆菌K-12的苏氨酸、亮氨酸和异亮氨酸操纵子施加调控。
J Bacteriol. 1983 Jul;155(1):49-55. doi: 10.1128/jb.155.1.49-55.1983.
10
A family of dispersed repetitive extragenic palindromic DNA sequences in E. coli.大肠杆菌中一类分散的重复基因外回文DNA序列家族。
EMBO J. 1984 Jun;3(6):1417-21. doi: 10.1002/j.1460-2075.1984.tb01986.x.
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Tryptophanyl-tRNA and tryptophanyl-tRNA synthetase are not required for in vitro repression of the tryptophan operon.色氨酸-tRNA和色氨酸-tRNA合成酶对于色氨酸操纵子的体外阻遏不是必需的。
Nat New Biol. 1973 Oct 3;245(144):131-3. doi: 10.1038/newbio245131a0.
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In vitro repression of transcription of the tryptophan operon by trp repressor.色氨酸阻遏物对色氨酸操纵子转录的体外抑制作用。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1990-4. doi: 10.1073/pnas.70.7.1990.
6
Interaction of the operator of the tryptophan operon with repressor.色氨酸操纵子的操纵基因与阻遏物的相互作用。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3134-8. doi: 10.1073/pnas.71.8.3134.
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Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli.大肠杆菌色氨酸操纵子阻遏蛋白的检测与分离
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Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function.色氨酸（trp）mRNA起始位点之前区域的核苷酸序列及其在启动子和操纵基因功能中的作用。
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2351-5. doi: 10.1073/pnas.73.7.2351.
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DNA sequence of the bacteriophage gama cI gene.噬菌体γ cI基因的DNA序列。
Nature. 1978 Nov 16;276(5685):301-2. doi: 10.1038/276301a0.
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Amino acid sequence of Cro regulatory protein of bacteriophage lambda.
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