色氨酸操纵子的操纵基因与阻遏物的相互作用。
Interaction of the operator of the tryptophan operon with repressor.
作者信息
Rose J K, Yanofsky C
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3134-8. doi: 10.1073/pnas.71.8.3134.
Abstract
Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo. The half-life of the trp repressor-trp operator complex is less than 2 min in vitro in 0.12 M salt.
摘要
对大肠杆菌色氨酸操纵子阻遏作用的体外转录研究表明，部分纯化的色氨酸阻遏物能特异性地结合含有色氨酸操纵基因的DNA，在37℃、0.12M盐浓度下，阻遏物与操纵基因的解离常数约为0.2 nM，这一数值与色氨酸操纵子在体内的调控程度相符。在0.12M盐浓度的体外环境中，色氨酸阻遏物 - 色氨酸操纵基因复合物的半衰期不到2分钟。
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Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan.在部分纯化的阻遏物和色氨酸存在的情况下，利用纯化的RNA聚合酶对色氨酸操纵子进行体外转录调控。
Nat New Biol. 1973 Oct 3;245(144):133-7. doi: 10.1038/newbio245133a0.
10
Tryptophanyl-tRNA and tryptophanyl-tRNA synthetase are not required for in vitro repression of the tryptophan operon.色氨酸-tRNA和色氨酸-tRNA合成酶对于色氨酸操纵子的体外阻遏不是必需的。
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