Padron J, Gallagher J T, Kent P W
Br J Exp Pathol. 1973 Aug;54(4):347-51.
Limited treatment with papain has been used to liberate isolated epithelial cells from the epithelial mucosa of the rabbit small intestine. Such cell populations retain their brush borders, have a Qo of -10 μl O/mg protein/hour and incorporate d[C]glucosamine into three high molecular weight components having certain electrophoretic properties characteristic of glycoproteins. This system should be valuable for studying glycoprotein metabolism in epithelial cells without interference from sub-epithelial tissues.
木瓜蛋白酶的有限处理已被用于从兔小肠上皮黏膜中分离出单个上皮细胞。这样的细胞群体保留了它们的刷状缘，Qo为-10 μl O/mg蛋白质/小时，并将d[C]葡萄糖胺掺入具有某些糖蛋白特征电泳特性的三种高分子量成分中。该系统对于研究上皮细胞中的糖蛋白代谢而不受上皮下组织的干扰应该是有价值的。
