Fjolstad M
Acta Vet Scand. 1973;14(1):69-80. doi: 10.1186/BF03547411.
Feeding experiments with Clostridium botulinum toxin type were carried out using 11 goats. The toxin, which originated partly from the liver of a cat carcass and partly from broth inoculated with Cl. botulinum type isolated from the cat liver, was given to the goats through a rubber stomach-tube. Symptoms of botulism usually occurred on the second or third day after the challenge. Death was caused by doses of toxin as small as 0.5 MMLD (minimum mouse lethal dose) per g body weight of goat, and 1 MMLD per g was considered to be lethal for goats when they are fed hay and concentrated fodder. When the animals were grazing or received ensilage they seemed to tolerate somewhat higher doses. A cumulative effect was shown to take place when the doses of toxin were given in the course of 8 days. A sensitizing effect of many small doses could not be demonstrated. On the contrary, animals which had received several small doses of toxin later on tolerated doses which caused death in inexperienced animals. One of these animals tolerated a dose approx. 3 times higher than a dose usually causing death. Serum from such goats, however, did not give demonstrable protection in mice challenged to botulinum toxin type . Toxin could not be demonstrated in samples of serum from goats showing symptoms of botulism. In 2 of 9 animals toxin could be demonstrated in samples from the liver, but only after the liver had remained in the carcass for approx. 20 hrs. after death.
用11只山羊进行了肉毒杆菌A型毒素的喂养实验。毒素部分来源于猫尸体的肝脏，部分来源于接种从猫肝脏分离出的肉毒杆菌A型的肉汤，通过橡胶胃管将毒素喂给山羊。肉毒中毒症状通常在注射毒素后的第二天或第三天出现。每克山羊体重注射低至0.5 MMLD（小鼠最小致死剂量）的毒素即可导致死亡，当给山羊喂干草和精饲料时，每克1 MMLD被认为对山羊具有致死性。当动物放牧或食用青贮饲料时，它们似乎能耐受稍高剂量的毒素。当在8天内给予毒素剂量时，显示出累积效应。未证明多次小剂量毒素具有致敏作用。相反，后来接受过几次小剂量毒素的动物能够耐受那些会导致未接触过毒素的动物死亡的剂量。其中一只动物耐受的剂量比通常导致死亡的剂量高出约3倍。然而，来自此类山羊的血清在受到肉毒杆菌A型毒素攻击的小鼠中并未表现出可证明的保护作用。在出现肉毒中毒症状的山羊的血清样本中未检测到毒素。在9只动物中的2只中，仅在肝脏在动物死后在尸体中留存约20小时后，才在肝脏样本中检测到毒素。
