Grogan W M, Scott R B, Collins J M
J Histochem Cytochem. 1979 Jun;27(6):1011-6. doi: 10.1177/27.6.458137.
A commerical cell sorter was used to obtain preparations of cells in various stages of granulocyte development from rabbit marrows stimulated by inflammatory response. Marrow cells were fractionated on density gradients of Ficoll/Hypaque and each fraction sorted using light scatter. Trial and error selection of appropriate gradient fractions and light scatter windows allowed sorting of early (blast cells, promyelocytes), intermediate (myelocytes, metamyelocytes) and late stage (band cells, polys) granulocytes with enhanced purity.
使用商用细胞分选仪从炎症反应刺激的兔骨髓中获取处于粒细胞发育不同阶段的细胞制剂。骨髓细胞在Ficoll/Hypaque密度梯度上进行分级分离，每个级分使用光散射进行分选。通过反复试验选择合适的梯度级分和光散射窗口，可以分选纯度更高的早期（原始细胞、早幼粒细胞）、中期（中幼粒细胞、晚幼粒细胞）和晚期（杆状核细胞、多形核细胞）粒细胞。
