Guerci O, Huot-Marchand F, Schneider O
Scand J Haematol. 1981 May;26(5):378-84. doi: 10.1111/j.1600-0609.1981.tb01678.x.
Sedimentation at unit gravity of human bone marrow cells, for 15 h at 4 degrees C on linear density gradient of Ficoll in culture medium ranging from 1.020 to 1.065 g/ml shows that a differential migration of the bone marrow cell sub-populations exists with precise mean densities 1.021 +/- 1 x 10(-3) g/ml for lymphocytes; 1.024 +/- 2.5 x 10(-3) g/ml for non-eosinophil granulocytes; and 1.055 +/- 10 x 10(-3) g/ml for metamyelocytes; 1.030 x 3.5 x 10(-3) g/ml for other myeloid cells (myeloblasts, promyelocytes, myelocytes); 1.040 +/- 1.040 +/- 3 x 10(-3) g/ml for eosinophil granulocytes; and 1.055 +/- 10 x 10(-3) g/ml for megakaryocytes. The highest percentages of S phase cell and G2 and M phase cells determined by a cytofluorograph correspond to the peaks of immature myeloid cells (myeloblasts, promyelocytes and myelocytes). This method of bone marrow cell separation may be used to study the cell cycle in pathological bone marrows (leukaemia in particular) and to determine the effects and the efficiency of some antimitotics.
在4℃条件下，将人骨髓细胞在密度为1.020至1.065 g/ml的Ficoll线性密度梯度培养基中进行单位重力沉降15小时，结果表明骨髓细胞亚群存在差异迁移，淋巴细胞的精确平均密度为1.021±1×10⁻³ g/ml；非嗜酸性粒细胞的平均密度为1.024±2.5×10⁻³ g/ml；晚幼粒细胞的平均密度为1.055±10×10⁻³ g/ml；其他髓系细胞（原粒细胞、早幼粒细胞、中幼粒细胞）的平均密度为1.030×3.5×10⁻³ g/ml；嗜酸性粒细胞的平均密度为1.040±1.040±3×10⁻³ g/ml；巨核细胞的平均密度为1.055±10×10⁻³ g/ml。通过细胞荧光分析仪测定的S期细胞以及G2和M期细胞的最高百分比对应于未成熟髓系细胞（原粒细胞、早幼粒细胞和中幼粒细胞）的峰值。这种骨髓细胞分离方法可用于研究病理性骨髓（尤其是白血病）中的细胞周期，并确定某些抗有丝分裂药物的作用和效果。
