Bagnara G P, Castaldini C, Minzi D, Brunelli M A
Boll Soc Ital Biol Sper. 1980 Jan 15;56(1):56-61.
Human normal marrow cells from 5 donors have been separated via discontinuous density gradient according to the Dicke's technique and the morphology of granulocytic cells recovered from density fractions has been examined; peroxidase negative myeloblasts are concentrated in low density fractions (specific density below 1.063), while granulated myeloblasts (peroxidase positive) are concentrated at densities ranging between 1.063 and 1.073; promyelocytes and myelocytes have been observed in all fractions although their maximal concentration is at density 1.073; metamyelocytes and polymorphonucleated cells are concentrated in the highest density fraction (density 1.078); some differentiated cells which are peroxidase negative have been observed; they concentrate at a density lower than that of peroxidase positive cells which look morphologically identical.
采用迪克技术，通过不连续密度梯度法对5名供体的人正常骨髓细胞进行了分离，并对从不同密度组分中回收的粒细胞形态进行了检查；过氧化物酶阴性的成髓细胞集中在低密度组分（比重低于1.063）中，而颗粒状成髓细胞（过氧化物酶阳性）则集中在比重为1.063至1.073的范围内；虽然早幼粒细胞和中幼粒细胞在所有组分中均有观察到，但其最大浓度出现在比重为1.073处；晚幼粒细胞和多形核细胞集中在最高密度组分（比重1.078）中；观察到一些过氧化物酶阴性的分化细胞；它们集中在比重低于形态上相同的过氧化物酶阳性细胞的密度处。
