Gilchrest B A
J Invest Dermatol. 1979 May;72(5):219-23. doi: 10.1111/1523-1747.ep12530769.
The relationship of actinically-induced "premature aging" to chronological aging was studied in paired keratinocyte cultures obtained from the habitually sun-exposed (lateral) and nonexposed (medial) aspects of the arm of 5 male donors, aged 41 to 80 yr. In all cases, the number of cell generations in vitro was greater for cultures derived from sun-exposed skin, and this discrepancy increased with donor age and the severity of clinical aging changes. Hence, chronic sun exposure does accelerate aging in human skin by at least one previously established in vitro criterion: it decreased the lifespan of cultured keratinocytes. Plating efficiency was 11- to 32-fold higher for keratinocytes from chronically sun-exposed skin than nonexposed controls, perhaps reflecting the recognized carcinogenic potential of actinic radiation. Keratinocyte cultures appear to be as amenable to gerontologic studies as the already widely used human fibroblast cultures.
在取自5名年龄在41至80岁男性供体手臂经常暴露于阳光的（外侧）和未暴露于阳光的（内侧）部位的配对角质形成细胞培养物中，研究了光诱导的“早衰”与自然衰老之间的关系。在所有情况下，来自暴露于阳光皮肤的培养物在体外的细胞传代数更多，并且这种差异随着供体年龄和临床衰老变化的严重程度而增加。因此，长期阳光照射至少通过一种先前确立的体外标准加速了人类皮肤的衰老：它缩短了培养角质形成细胞的寿命。长期暴露于阳光的皮肤角质形成细胞的接种效率比未暴露的对照高11至32倍，这可能反映了光辐射公认的致癌潜力。角质形成细胞培养物似乎与已经广泛使用的人类成纤维细胞培养物一样适合进行老年学研究。
