Gilchrest B A
J Gerontol. 1980 Jul;35(4):537-41. doi: 10.1093/geronj/35.4.537.
The relationship of actinically-induced "premature aging" to chronologic aging was studied in paired fibroblast cultures obtained from the habitually sun-exposed (lateral) and nonexposed (medial) aspects of the arm of eight male donors, aged 41 to 80 years. In each case, the fibroblast strain derived from the medial, nonexposed aspect of the arm underwent more cumulative population doublings than did the paired strain from the lateral sun-exposed aspect, and this discrepancy increased with donor age and severity of clinical aging changes. Hence, chronic sun exposure does accelerate aging in human skin by at least one established in vitro criterion: it decreases the lifespan of cultured fibroblasts. The data underline the difficulty of distinguishing environmental effects from intrinsic aging changes.
在取自8名年龄在41至80岁男性供体手臂上经常暴露于阳光下的（外侧）和未暴露于阳光下的（内侧）部位的成对成纤维细胞培养物中，研究了光诱导的“早衰”与自然衰老之间的关系。在每种情况下，源自手臂内侧未暴露部位的成纤维细胞系比来自外侧暴露于阳光下的成对细胞系经历了更多的累积群体倍增，并且这种差异随着供体年龄和临床衰老变化的严重程度而增加。因此，长期阳光照射至少通过一种既定的体外标准加速了人类皮肤的衰老：它缩短了培养的成纤维细胞的寿命。这些数据强调了区分环境影响和内在衰老变化的困难。
