Marks M W, Morykwas M J, Wheatley M J
Department of Plastic and Reconstructive Surgery, Bowman Gray School of Medicine, Winston, Salem, N.C.
Plast Reconstr Surg. 1990 Aug;86(2):255-9. doi: 10.1097/00006534-199008000-00008.
The changes in skin morphology over time are a consequence of both chronologic aging and the accumulation of environmental exposure. Through observation, we know that actinic radiation intensifies the apparent aging of skin. We have investigated the effects of aging and actinic radiation on the ability of fibroblasts to contract collagen-fibroblast lattices. Preauricular and postauricular skin samples were obtained from eight patients aged 49 to 74 undergoing rhytidectomy. The samples were kept separate, and the fibroblasts were grown in culture. Lattices constructed with preauricular fibroblasts consistently contracted more than lattices containing postauricular fibroblasts. The difference in amount of contraction in 7 days between sites was greatest for the younger patients and decreased linearly as donor age increased (r = -0.96). This difference may be due to preauricular fibroblasts losing their ability to contract a lattice as aging skin is exposed to more actinic radiation.
随着时间推移，皮肤形态的变化是自然衰老和环境暴露积累共同作用的结果。通过观察，我们了解到光化辐射会加剧皮肤的表观老化。我们研究了衰老和光化辐射对成纤维细胞收缩胶原-成纤维细胞网格能力的影响。从8例年龄在49至74岁接受除皱手术的患者身上获取耳前和耳后皮肤样本。样本分开保存，成纤维细胞在培养中生长。用耳前成纤维细胞构建的网格收缩程度始终大于含耳后成纤维细胞的网格。7天内不同部位收缩量的差异在年轻患者中最大，并随着供体年龄的增加呈线性下降（r = -0.96）。这种差异可能是由于随着老化皮肤暴露于更多的光化辐射，耳前成纤维细胞失去了收缩网格的能力。
