Tani Y, Dempsey W B
J Bacteriol. 1973 Oct;116(1):341-5. doi: 10.1128/jb.116.1.341-345.1973.
Carbon-labeled glycolaldehyde prepared from [(14)C]serine was used to supply the nutritional requirement of a pyridoxineless auxotroph of Escherichia coli. Pyridoxal phosphate isolated from bacteria so grown was found to have incorporated the radioactive glycolaldehyde with little dilution. The radioactivity which was unincorporated into pyridoxal phosphate was recovered almost entirely in the culture fluid. The results establish for the first time that glycolaldehyde is indeed a natural precursor of pyridoxal phosphate or it is readily converted to such a precursor.
由[¹⁴C]丝氨酸制备的碳标记乙醇醛用于满足大肠杆菌无维生素B₆营养缺陷型的营养需求。从这样培养的细菌中分离出的磷酸吡哆醛被发现掺入了放射性乙醇醛，且稀释很少。未掺入磷酸吡哆醛的放射性几乎完全在培养液中回收。这些结果首次证实乙醇醛确实是磷酸吡哆醛的天然前体，或者它很容易转化为这样一种前体。
