用于核糖核酸聚合酶基因的phi80转导噬菌体的分离与鉴定
Isolation and characterization of phi80 transducing bacteriophage for a ribonucleic acid polymerase gene.
作者信息
Konrad B, Kirschbaum J, Austin S
出版信息
J Bacteriol. 1973 Nov;116(2):511-6. doi: 10.1128/jb.116.2.511-516.1973.
Abstract
A new method for isolating specialized transducing phages is described. It was used to isolate a group of phi80 transducing phages which carry various bacterial markers from the metB region of the Escherichia coli chromosome. Some of the phages selected for transduction of the supA36 marker were also shown to carry rif, a locus known to specify the beta subunit of ribonucleic acid polymerase. Expression of the prophage rif(r) gene in lysogens was demonstrated by its ability to confer rifampin resistance on part of the cellular ribonucleic acid polymerase pool.
摘要
本文描述了一种分离特异性转导噬菌体的新方法。该方法用于分离一组携带大肠杆菌染色体metB区域各种细菌标记的phi80转导噬菌体。一些被选择用于转导supA36标记的噬菌体也被证明携带rif，该位点已知可指定核糖核酸聚合酶的β亚基。原噬菌体rif(r)基因在溶源菌中的表达通过其赋予部分细胞核糖核酸聚合酶库利福平抗性的能力得以证明。
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