Krueger J H, Johnson J R, Greene R C, Dresser M
J Bacteriol. 1981 Aug;147(2):612-21. doi: 10.1128/jb.147.2.612-621.1981.
The structures of several lambda dmet and related lambda darg transducing phage were studied by restriction fragment mapping and electron microscopic measurements of homoduplexes and heteroduplexes. A new transducing phage (lambda dmet141), in which metF is the only functional gene of the cluster, was isolated. In contrast, lambda dmet117, which expresses the entire metBJLF cluster, has only 3 kilobases more bacterial deoxyribonucleic acid (DNA) than lambda dmet141. An EcoRI restriction fragment of lambda dmet117, which carries the leftmost 6 kilobases of the bacterial DNA insert, was isolated and shown to contain a functional copy of metB. Small structural differences at the attachment sites of some of the phage were shown to result from different sites of lambda integration in the two parent insertion lysogens.
通过限制性片段图谱分析以及同源双链体和异源双链体的电子显微镜测量，对几种λdmet及相关的λdarg转导噬菌体的结构进行了研究。分离出一种新的转导噬菌体（λdmet141），其中metF是该基因簇中唯一的功能基因。相比之下，表达整个metBJLF基因簇的λdmet117比λdmet141仅多3千碱基的细菌脱氧核糖核酸（DNA）。分离出λdmet117的一个EcoRI限制性片段，它携带细菌DNA插入片段最左边的6千碱基，并显示其含有metB的功能拷贝。结果表明，某些噬菌体附着位点的微小结构差异是由λ在两个亲本插入溶原菌中的整合位点不同所致。
