乳糖阻遏蛋白：用于核磁共振研究的3-氟酪氨酸取代
lac repressor: 3-fluorotyrosine substitution for nuclear magnetic resonance studies.
作者信息
Lu P, Jarema M, Mosser K, Daniel W E
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3471-5. doi: 10.1073/pnas.73.10.3471.
Abstract
This paper describes the isolation of 3-fluorotyrosine-substituted lac repressor, and its 19F nuclear magnetic resonance spectrum. From the spectrum, one can conclude that for each of the four identical subunits of the repressor there are four or five surface tyrosines, two buried or internal tyrosines, and one tyrosine with an phenolic group ionized or involved in a hydrogen bond. Conditions are described that can be used for the 3-fluorotyrosine substitution of a variety of Escherichia coli proteins for 19F nuclear magnetic resonance studies.
摘要
本文描述了3-氟酪氨酸取代的乳糖阻遏物的分离及其19F核磁共振谱。从该谱图可以得出结论，对于阻遏物的四个相同亚基中的每一个，有四个或五个表面酪氨酸、两个埋藏或内部酪氨酸，以及一个酚基离子化或参与氢键形成的酪氨酸。文中还描述了可用于多种大肠杆菌蛋白质的3-氟酪氨酸取代以进行19F核磁共振研究的条件。
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Proc Natl Acad Sci U S A. 1976 Oct;73(10):3471-5. doi: 10.1073/pnas.73.10.3471.
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Regulation of 3-deoxy-D-arabino-heptulosonic 7-phosphate acid synthetase activity in relation to the synthesis of the aromatic vitamins in Escherichia coli K-12.大肠杆菌K-12中3-脱氧-D-阿拉伯庚酮糖-7-磷酸合成酶活性与芳香族维生素合成的关系调控
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Sequence analysis of fluorescamine-stained peptides and proteins purified on a nanomole scale. Application to proteins of bacteriophage MS2.对荧光胺染色的、在纳摩尔规模上纯化的肽和蛋白质进行序列分析。应用于噬菌体MS2的蛋白质。
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10
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