大肠杆菌K-12温度敏感型膜突变体的分离
Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12.
作者信息
Steenbakkers J F, Broekman J H, Kerkenaar A, de Haan P G
出版信息
J Bacteriol. 1973 Nov;116(2):535-40. doi: 10.1128/jb.116.2.535-540.1973.
Abstract
Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.
摘要
从不饱和脂肪酸生物合成受损或磷脂代谢改变的一组温度敏感型裂解突变体中，以相当高的频率分离出了突变体。有人提出，对裂解表型进行预筛选使得分离出几种影响细胞质膜完整性的突变体成为可能。
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