Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12.
作者信息
Steenbakkers J F, Broekman J H, Kerkenaar A, de Haan P G
出版信息
J Bacteriol. 1973 Nov;116(2):535-40. doi: 10.1128/jb.116.2.535-540.1973.
Abstract
Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.
摘要
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