大肠杆菌K - 12的不饱和脂肪酸营养缺陷型在高渗培养基中的生长。
Growth in high osmotic medium of an unsaturated fatty acid auxotroph of Escherichia coli K-12.
作者信息
Broekman J H, Steenbakkers J F
出版信息
J Bacteriol. 1973 Oct;116(1):285-9. doi: 10.1128/jb.116.1.285-289.1973.
Abstract
A temperature-sensitive mutant of Escherichia coli K-12, which is impaired in the biosynthesis of the unsaturated fatty acids at 42 C, continues to grow and remains viable in a medium with a high osmotic pressure. Under these growth conditions, 50% of the fatty acids of the parental strain and 80% of the fatty acids of the mutant strain are saturated.
摘要
大肠杆菌K-12的一种温度敏感突变体，在42℃时不饱和脂肪酸生物合成受损，但在高渗透压培养基中仍能继续生长并保持存活。在这些生长条件下，亲代菌株50%的脂肪酸和突变菌株80%的脂肪酸是饱和的。
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本文引用的文献
1
EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI.温度对大肠杆菌脂肪酸组成的影响
J Bacteriol. 1962 Dec;84(6):1260-7. doi: 10.1128/jb.84.6.1260-1267.1962.
2
Mutation of bacteria at high levels of survival by ethyl methane sulphonate.用甲磺酸乙酯使细菌在高存活水平下发生突变。
Nature. 1959 Dec 5;184:1780-2. doi: 10.1038/1841780a0.
3
Monomolecular layers of synthetic phosphatides.合成磷脂单分子层。
J Pharm Pharmacol. 1962 Jul;14:429-44. doi: 10.1111/j.2042-7158.1962.tb11121.x.
4
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
5
The influence of NaCl concentration of the medium on the potassium content of Aerobacter aerogenes and on the inter-relationships between potassium, magnesium and ribonucleic acid in the growing bacteria.培养基中氯化钠浓度对产气气杆菌钾含量以及生长中细菌体内钾、镁和核糖核酸之间相互关系的影响。
J Gen Microbiol. 1968 Dec;54(3):319-25. doi: 10.1099/00221287-54-3-319.
6
Lipid composition and permeability of liposomes.脂质体的脂质组成与通透性
Biochim Biophys Acta. 1968 Jun 11;150(4):666-75. doi: 10.1016/0005-2736(68)90056-4.
7
Mapping of a locus for unsaturated fatty acid biosynthesis in Escherichia coli.大肠杆菌中不饱和脂肪酸生物合成基因座的定位
J Bacteriol. 1970 Jul;103(1):273-4. doi: 10.1128/jb.103.1.273-274.1970.
8
Control of fatty acid composition in phospholipids of Escherichia coli: response to fatty acid supplements in a fatty acid auxotroph.大肠杆菌磷脂中脂肪酸组成的调控：脂肪酸营养缺陷型对脂肪酸补充物的反应
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1057-64. doi: 10.1073/pnas.64.3.1057.
9
Studies on the mechanism of fatty acid synthesis. XXII. Salt activation of the fatty acid-synthesizing enzymes of Escherichia coli.脂肪酸合成机制的研究。第二十二部分。大肠杆菌脂肪酸合成酶的盐激活作用。
J Biol Chem. 1969 Dec 25;244(24):6577-83.
10
Fatty acid replacements in a fatty acid auxotroph of Escherichia coli.大肠杆菌脂肪酸营养缺陷型中的脂肪酸替代物。
J Bacteriol. 1968 May;95(5):1658-65. doi: 10.1128/jb.95.5.1658-1665.1968.
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