Hurmerinta K, Thesleff I, Saxén L
J Embryol Exp Morphol. 1979 Apr;50:99-109.
Molar tooth germs from mouse embryos were studied in a Trowell-type organ culture. After 5 days of culture the odontoblasts had secreted predentine and the ameloblasts had differentiated. When cultured in the presence of 10-50 micro M diazo-oxo-norleucine (DON), which is a glutamine analogue, the differentiation of odontoblasts was inhibited, but the teeth looked otherwise healthy. When DON was added after 2 days of culture in control medium (at this time the odontoblasts in the cuspal area were already differentiated), it did not inhibit predentine secretion, ameloblast differentiation, nor enamel secretion. However, this was seen only in the cuspal area and the boundary to the undifferentiated, more cervical cells was distinct. The results support the concept that the mechanism of the differentiation of odontoblasts is different from that of the ameloblasts. We have shown earlier that a close association between the basement membrane and the mesenchymal cells is required for odontoblast differentiation. Because DON interferes with glycosaminoglycan and glycoprotein synthesis we suggest that DON inhibits odontoblast differentiation by affecting the mesenchymal cell surface and/or the basement membrane.
在特罗韦尔型器官培养中研究了来自小鼠胚胎的磨牙牙胚。培养5天后，成牙本质细胞分泌了前期牙本质，成釉细胞已分化。当在10 - 50微摩尔重氮氧代正亮氨酸（DON，一种谷氨酰胺类似物）存在的情况下培养时，成牙本质细胞的分化受到抑制，但牙齿在其他方面看起来健康。当在对照培养基中培养2天后添加DON（此时牙尖区域的成牙本质细胞已经分化）时，它并不抑制前期牙本质的分泌、成釉细胞的分化或釉质的分泌。然而，这仅在牙尖区域可见，并且与未分化的、更靠近颈部的细胞之间的边界清晰。结果支持了成牙本质细胞的分化机制与成釉细胞不同的概念。我们之前已经表明，成牙本质细胞分化需要基底膜与间充质细胞之间紧密关联。因为DON干扰糖胺聚糖和糖蛋白合成，我们认为DON通过影响间充质细胞表面和/或基底膜来抑制成牙本质细胞的分化。
