从大肠杆菌的R⁺和ColV2⁺菌株中分离高频重组（Hfr）菌株，并通过转导获得R'lac因子。
Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction.
作者信息
Nishimura A, Nishimura Y, Caro L
出版信息
J Bacteriol. 1973 Dec;116(3):1107-12. doi: 10.1128/jb.116.3.1107-1112.1973.
Abstract
Temperature-resistant revertants were isolated from Escherichia coli strains carrying a temperature-sensitive dnaA mutation (initiation of chromosome replication) and either a repressed or a derepressed F-like R factor or a ColV2 factor. Many of the revertants had all the properties of Hfr strains, with a variety of directions and origins of transfer. From one such revertant, episomes carrying the R factor and part of the lac region (R'lac) could be isolated by transduction. This system offers a good selection for Hfr strains produced by integration of various episomes and for the isolation of R' factors.
摘要
从携带温度敏感型dnaA突变（染色体复制起始）以及受抑制或去抑制的F样R因子或ColV2因子的大肠杆菌菌株中分离出耐温回复突变体。许多回复突变体具有Hfr菌株的所有特性，具有多种转移方向和起源。从一个这样的回复突变体中，可以通过转导分离出携带R因子和部分lac区域（R'lac）的附加体。该系统为通过各种附加体整合产生的Hfr菌株以及R'因子的分离提供了良好的选择。
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