Sutfin T A, Jusko W J
J Pharm Sci. 1979 Jun;68(6):703-5. doi: 10.1002/jps.2600680612.
High-performance liquid chromatographic method is presented for the simultaneous determination of imipramine, desipramine, and their 2-hydroxylated metabolites in plasma. The method involves a simple plasma extraction at basic pH with organic solvent, chromatography on a silica gel column, and fluorescence detection. Correlation with a GLC-mass spectrometric method for imipramine and desipramine is illustrated. The method can detect 1 ng of each component/ml of plasma, sufficient sensitivity for pharmocokinetic studies.
本文介绍了一种高效液相色谱法，用于同时测定血浆中的丙咪嗪、地昔帕明及其2-羟基化代谢产物。该方法包括在碱性pH条件下用有机溶剂对血浆进行简单萃取、在硅胶柱上进行色谱分析以及荧光检测。文中展示了该方法与用于丙咪嗪和地昔帕明的气相色谱-质谱法的相关性。该方法能够检测出血浆中每毫升1纳克的各组分，对药代动力学研究而言具有足够的灵敏度。
