Vinther O, Freundt E A
J Bacteriol. 1980 May;142(2):683-8. doi: 10.1128/jb.142.2.683-688.1980.
The transverse disposition of exposed protein antigens on the two faces of isolated Mycoplasma gallisepticum membranes have been investigated by using indirect immunoferritin labeling to accomplish visualization of the antigens at the ultrastructural level. Comparison between the labeling patterns obtained with unabsorbed specific mycoplasma antiserum and antiserum from which antibodies directed against outer side determinants had been removed revealed that the majority of protein antigens were the same on the opposed membrance faces or at least displayed extensive interside cross-reactivity. The relatively scarce tagging of isolated Acholeplasma laidlawii membranes, contrary to membranes on intact organisms observed in this investigation, precluded conclusions regarding the disposition of membrane antigens of this species. The advantages and limitations of the employed method in disposition studies and the factors influencing the transverse distribution of membrane proteins in mycoplasmas are discussed.
通过使用间接免疫铁蛋白标记法在超微结构水平上实现抗原可视化，对鸡毒支原体分离膜两面暴露的蛋白质抗原的横向分布进行了研究。用未吸收的特异性支原体抗血清和已去除针对外侧决定簇抗体的抗血清获得的标记模式进行比较，结果显示，大多数蛋白质抗原在相对的膜面上是相同的，或者至少表现出广泛的跨面交叉反应性。与本研究中观察到的完整生物体上的膜相反，莱氏无胆甾原体分离膜的标记相对较少，这使得无法得出关于该物种膜抗原分布的结论。讨论了所采用方法在分布研究中的优缺点以及影响支原体膜蛋白横向分布的因素。
