对单个活细胞的一部分进行温和、差异加热的方法。
Methods for gentle, differential heating of part of a single living cell.
作者信息
Nicklas R B
出版信息
J Cell Biol. 1973 Dec;59(3):595-600. doi: 10.1083/jcb.59.3.595.
Abstract
The fabrication and use of resistance wire microheaters is described and the results obtainable with cells are illustrated. The microheaters permit localized warming of a small area, wherever desired, within a single living cell. This is directly shown by the production of localized structural changes in the mitotic spindle. Thus in this case, subcellular areas respond independently to the local temperature. The method is applicable to a variety of cellular processes, providing clues to cellular control mechanisms.
摘要
本文描述了电阻丝微加热器的制作与使用，并展示了在细胞上可获得的结果。这些微加热器能够在单个活细胞内的任何所需位置实现小区域的局部加热。有丝分裂纺锤体中局部结构变化的产生直接证明了这一点。因此，在这种情况下，亚细胞区域对局部温度独立作出反应。该方法适用于多种细胞过程，为细胞控制机制提供了线索。
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本文引用的文献
1
CONSTRUCTION OF MICRO-THERMOCOUPLES.微型热电偶的构建
Science. 1929 Sep 13;70(1811):263-6. doi: 10.1126/science.70.1811.263.
2
Surface characters of dividing cells. 3. Unequal division caused by steep temperature gradient in grasshopper spermatocyte.
Dev Growth Differ. 1969 Oct;11(2):104-14. doi: 10.1111/j.1440-169x.1969.00104.x.
3
Chromosome micromanipulation. I. The mechanics of chromosome attachment to the spindle.染色体显微操作。I. 染色体附着于纺锤体的机制。
Chromosoma. 1967;21(1):1-16. doi: 10.1007/BF00330544.
4
How microtubule patterns are generated. The relative importance of nucleation and bridging of microtubules in the formation of the axoneme of Raphidiophrys.微管模式是如何形成的。微管的成核作用和桥接作用在Raphidiophrys轴丝形成中的相对重要性。
J Cell Biol. 1971 Dec;51(3):837-54. doi: 10.1083/jcb.51.3.837.
5
[Microtubule distribution in metaphase and anaphase spindles of the spermatocytes of Pales ferruginea. A quantitative analysis of serial cross-sections (author's transl)].[赤松毛虫精母细胞中期和后期纺锤体中的微管分布。连续横断面的定量分析（作者译）]
Chromosoma. 1973 Aug 10;43(2):109-43. doi: 10.1007/BF00483375.
6
A thermodynamic analysis of mitotic spindle equilibrium at active metaphase.活跃中期有丝分裂纺锤体平衡的热力学分析。
J Cell Biol. 1973 Apr;57(1):133-47. doi: 10.1083/jcb.57.1.133.
7
Partial cell irradiation with a tunable organic dye laser.用可调谐有机染料激光进行细胞局部照射。
Nature. 1972 Dec 22;240(5382):483-5. doi: 10.1038/240483a0.
8
Influence of autosome movements and of sex-chromosome movements on sex-chromosome segregation in crane fly spermatocytes.
Chromosoma. 1973;40(4):417-42. doi: 10.1007/BF00399432.
9
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Int Rev Cytol. 1972;33:131-56. doi: 10.1016/s0074-7696(08)61450-3.
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