对单个活细胞的一部分进行温和、差异加热的方法。
Methods for gentle, differential heating of part of a single living cell.
作者信息
Nicklas R B
出版信息
J Cell Biol. 1973 Dec;59(3):595-600. doi: 10.1083/jcb.59.3.595.
Abstract
The fabrication and use of resistance wire microheaters is described and the results obtainable with cells are illustrated. The microheaters permit localized warming of a small area, wherever desired, within a single living cell. This is directly shown by the production of localized structural changes in the mitotic spindle. Thus in this case, subcellular areas respond independently to the local temperature. The method is applicable to a variety of cellular processes, providing clues to cellular control mechanisms.
摘要
本文描述了电阻丝微加热器的制作与使用，并展示了在细胞上可获得的结果。这些微加热器能够在单个活细胞内的任何所需位置实现小区域的局部加热。有丝分裂纺锤体中局部结构变化的产生直接证明了这一点。因此，在这种情况下，亚细胞区域对局部温度独立作出反应。该方法适用于多种细胞过程，为细胞控制机制提供了线索。
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