逆流过滤技术在海洋微生物研究中的适用性。
Applicability of the reverse-flow filter technique to marine microbial studies.
作者信息
Griffiths R P, Hanus F J, Morita R Y
出版信息
Appl Microbiol. 1973 Nov;26(5):687-91. doi: 10.1128/am.26.5.687-691.1973.
Abstract
The use of the reverse-flow filtration technique to quantitatively concentrate marine bacteria was evaluated using both a pure culture and seawater samples. Our data indicate that the cells are altered during the filtration procedure and that a significant and inconsistent number of cells are lost on the membrane filter. The results obtained indicate that data on marine bacteria concentrated in this manner should be interpreted with caution.
摘要
使用逆流过滤技术对海洋细菌进行定量浓缩，分别在纯培养物和海水样本上进行了评估。我们的数据表明，细胞在过滤过程中发生了改变，并且有相当数量且不一致的细胞在膜滤器上损失。所得结果表明，以这种方式浓缩的海洋细菌数据在解释时应谨慎。
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