副溶血性弧菌计数的膜过滤法
Membrane filter procedure for enumeration of Vibrio parahaemolyticus.
作者信息
Watkins W D, Thomas C D, Cabelli V J
出版信息
Appl Environ Microbiol. 1976 Nov;32(5):679-84. doi: 10.1128/aem.32.5.679-684.1976.
Abstract
A membrane filtration procedure has been developed for the enumeration of Vibrio parahaemolyticus in marine waters. Background microbial growth on the primary medium was decreased through the use of sodium cholate and copper sulfate, high pH, 3% NaCl, and an elevated incubation temperature. A series of in situ tests was employed to obviate the picking of colonies for identification; thereby, the enumeration of V. parahaemolyticus was accomplished within 30 h. Confirmation of typical colonies approached 95%. Relative to immediate plating on brain heart infusion agar spread plates, the recovery of V. parahaemolyticus cells suspended in phosphate-buffered saline or in seawater held for 24 h at 4 to 6 degrees C was about 90%. Assay variability did not exceed that expected by chance. Recoveries of V. parahaemolyticus from coastal and estuarine surface waters exceeded those obtainable by other methods examined.
摘要
已开发出一种膜过滤程序用于对海水中副溶血性弧菌进行计数。通过使用胆酸钠、硫酸铜、高pH值、3%氯化钠以及提高培养温度，减少了基础培养基上的背景微生物生长。采用了一系列原位测试以避免挑选菌落进行鉴定；从而在30小时内完成了副溶血性弧菌的计数。典型菌落的确认率接近95%。相对于立即接种在脑心浸液琼脂平板上，悬浮于磷酸盐缓冲盐水或在4至6摄氏度下保存24小时的海水中的副溶血性弧菌细胞的回收率约为90%。检测变异性未超过偶然预期的范围。从沿海和河口表层水中回收副溶血性弧菌的效率超过了所检测的其他方法。
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