用于铜绿假单胞菌计数的膜过滤技术
Membrane filter technique for enumeration of Pseudomonas aeruginosa.
作者信息
Levin M A, Cabelli V J
出版信息
Appl Microbiol. 1972 Dec;24(6):864-70. doi: 10.1128/am.24.6.864-870.1972.
Abstract
A membrane filter procedure for the quantitation of Pseudomonas aeruginosa (mPA procedure) has been developed. Through the use of inhibitors and an elevated incubation temperature, the level of background microbial flora was decreased approximately 10,000-fold. Using P. aeruginosa cells suspended in sea water and held for 24 hr, between 70 and 100% of the "viable" cells could be recovered by the mPA procedure. Assay variability was found to be insignificant. The recoveries of P. aeruginosa from surface (fresh and salt) waters, potable waters, and sewage by the mPA procedure exceeded those obtainable by current methods. Subsequent to its development and evaluation, the mPA procedure was used at three other laboratories for the enumeration of P. aeruginosa in potable and recreational waters and in sewage samples. It was found amenable to routine use, and confirmation of typical colonies approached 100%.
摘要
已开发出一种用于定量铜绿假单胞菌的膜过滤程序（mPA程序）。通过使用抑制剂和提高培养温度，背景微生物菌群水平降低了约10000倍。使用悬浮在海水中并保存24小时的铜绿假单胞菌细胞，通过mPA程序可回收70%至100%的“活”细胞。发现测定变异性不显著。通过mPA程序从地表水（淡水和咸水）、饮用水和污水中回收铜绿假单胞菌的效率超过了现有方法。在开发和评估之后，mPA程序在其他三个实验室用于饮用水、娱乐用水和污水样本中铜绿假单胞菌的计数。发现它适合常规使用，典型菌落的确认率接近100%。
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