Vossen J M, de Koning J, van Bekkum D W, Dicke K A, Eysvoogel V P, Hijmans W, van Loghem E, Rádl J, van Rood J J, van der Waay D, Dooren L J
Clin Exp Immunol. 1973 Jan;13(1):9-20.
A 4½-month-old boy suffering from congenital severe combined immunodeficiency was successfully treated by transplantation of bone marrow-derived cells. His parents were cousins in the first degree. The donor was a 32-year-old maternal uncle, who was HL-A genotypically identical with the patient as was shown by serological typing and MLC. A stem cell rich fraction of the donor's bone marrow was prepared by albumin gradient centrifugation. The infant showed a full immunological reconstitution without any sign of GVH disease. Remarkably the only allotypic marker of his IgG which was different from the donor allotype remained in the serum after transplantation, and even showed an increase of its level. The infant was nursed in strict reverse isolation and his unfavourable endogenous microflora of high potential pathogenicity was eliminated by antibiotic decontamination.
一名患有先天性严重联合免疫缺陷的4个半月大男孩通过移植骨髓来源的细胞成功得到治疗。他的父母是一级表亲。供体是一名32岁的舅舅，血清学分型和混合淋巴细胞培养显示其HL-A基因型与患者完全相同。通过白蛋白梯度离心法制备了供体骨髓中富含干细胞的部分。婴儿实现了完全的免疫重建，没有任何移植物抗宿主病的迹象。值得注意的是，移植后其血清中唯一与供体异型不同的IgG同种异型标记物仍然存在，甚至其水平还有所升高。婴儿在严格的反向隔离中护理，通过抗生素去污消除了具有高潜在致病性的不良内源性微生物群。
