Mitrovic D, Lippiello L, Mankin H J
J Rheumatol. 1979 Mar-Apr;6(2):124-30.
Chondrocytes isolated from calf articular cartilage by pretreatment with various enzymes prior to collagenase digestion were examined to determine the effect of enzymatic exposure on cell viability and to establish optimal conditions whereby freshly isolated cells could be used for short term metabolic studies. Pre-collagenase exposure to proteolytic enzymes for short intervals has no effect on cell viability. Trypsin pre-treatment appeared to increase the efficiency of cell isolation. Proteoglycan and RNA synthetic activity was influenced by the conditions of incubation (i.e., cell suspension vs. cell pellet: with or without a 24 hour "rest" period; and differential pre-collagenase enzyme exposure). The data suggest that the experimental conditions of incubation and the enzymes used in isolation of the cells, materially alter the freshly isolated chondrocyte's "pattern" of metabolic activity in short term culture studies.
通过在胶原酶消化之前用各种酶进行预处理从小牛关节软骨中分离出软骨细胞，以确定酶处理对细胞活力的影响，并建立最佳条件，以便将新鲜分离的细胞用于短期代谢研究。在胶原酶处理之前短时间暴露于蛋白水解酶对细胞活力没有影响。胰蛋白酶预处理似乎提高了细胞分离效率。蛋白聚糖和RNA合成活性受孵育条件（即细胞悬液与细胞沉淀：有无24小时“静置”期；以及胶原酶处理前不同的酶暴露情况）影响。数据表明，在短期培养研究中，孵育的实验条件和细胞分离中使用的酶会实质性地改变新鲜分离的软骨细胞的代谢活性“模式”。
