Reinholt F P, Wikström B, Hjerpe A, Engfeldt B
Eur J Cell Biol. 1984 Mar;33(2):229-33.
Chondrocytes were isolated from costal cartilage in young rats after digestion with collagenase and hyaluronidase. The immediate survival of the cells was investigated with the use of different criteria for viability, namely structural integrity and metabolic activity. Structural integrity was studied by transmission and scanning electron microscopy, trypan blue exclusion and NADH oxidation. Metabolic activity was measured both as O2 consumption and as proline and sulphate incorporation, as indicators of collagen and proteoglycan synthesis. The cellular content of glutathione was also measured. The chondrocytes isolated were found to be structurally intact and metabolically active. Early after isolation the chondrocytes varied considerably in size similarly to the native tissue. A selective loss of the larger sized cells was observed during further incubation for 24 h.
用胶原酶和透明质酸酶消化后，从幼鼠的肋软骨中分离出软骨细胞。使用不同的活力标准，即结构完整性和代谢活性，来研究细胞的即时存活率。通过透射电子显微镜、扫描电子显微镜、台盼蓝排斥试验和NADH氧化来研究结构完整性。以氧气消耗以及脯氨酸和硫酸盐掺入量作为胶原和蛋白聚糖合成的指标来测量代谢活性。还测量了谷胱甘肽的细胞含量。发现分离出的软骨细胞结构完整且代谢活跃。分离后早期，软骨细胞的大小与天然组织相似，差异很大。在进一步培养24小时期间，观察到较大尺寸的细胞有选择性损失。
