Spicer S S, Brissie R M, Thompson N T
Am J Pathol. 1975 Jun;79(3):481-98.
The Verhoeff iron hematoxylin-lead citrate (VIH-LC) method demonstrated vertical elastic fibers that were often composed only of microfibrillar component extending into the epidermal basement membrane in human skin. These fibers connected with a network of trabeculae composed of microfibrils and elastin fibrils in varying proportions. The large elastic fibers in the deep two thirds of the dermis consited mainly of compact bundles of small elstin fibrils in infants and of solid elastin cores with a fimbriated periphery in adults; Dermis of a 6-month-old fetus contained very few small elastic fibrils except around blood vessels. Skin of an elderly subject revealed exteme proliferation of unusual reticulated elastic fibers in various areas and disclosed abnormal nodules of elastin or collagen fibrils in finely particulate matter. Small elastin fibrils, abundant microfibrils, and intermixed individual collagen fibrils comprised an adventitial collar between sweat glands and fibroblasts. Elastin fibrils were absent from this collar in the fetus and increased with the subject's age. A permanganate-high iron diamine sequence appeared to impart density to the microfibrillar component of elastic fibers.
魏尔啸铁苏木精-柠檬酸盐铅（VIH-LC）法显示，在人体皮肤中，垂直弹性纤维通常仅由延伸至表皮基底膜的微原纤维成分组成。这些纤维与由微原纤维和弹性纤维按不同比例组成的小梁网络相连。婴儿真皮深层三分之二的大弹性纤维主要由紧密排列的小弹性纤维束组成，而成人则由具有边缘呈流苏状的实心弹性蛋白核心组成；6个月大胎儿的真皮除血管周围外，几乎没有小弹性纤维。老年受试者的皮肤在各个区域显示出异常网状弹性纤维的极度增生，并在细颗粒物质中发现弹性蛋白或胶原纤维的异常结节。小弹性纤维、丰富的微原纤维和混合的单个胶原纤维构成了汗腺和成纤维细胞之间的外膜环。胎儿的这个环中没有弹性纤维，且随着受试者年龄的增长而增加。高锰酸盐-高铁二胺序列似乎使弹性纤维的微原纤维成分具有密度。
