Variability of dermal elastin visualized ultrastructurally with iron hematoxylin.

The Verhoeff iron hematoxylin-lead citrate (VIH-LC) method demonstrated vertical elastic fibers that were often composed only of microfibrillar component extending into the epidermal basement membrane in human skin. These fibers connected with a network of trabeculae composed of microfibrils and elastin fibrils in varying proportions. The large elastic fibers in the deep two thirds of the dermis consited mainly of compact bundles of small elstin fibrils in infants and of solid elastin cores with a fimbriated periphery in adults; Dermis of a 6-month-old fetus contained very few small elastic fibrils except around blood vessels. Skin of an elderly subject revealed exteme proliferation of unusual reticulated elastic fibers in various areas and disclosed abnormal nodules of elastin or collagen fibrils in finely particulate matter. Small elastin fibrils, abundant microfibrils, and intermixed individual collagen fibrils comprised an adventitial collar between sweat glands and fibroblasts. Elastin fibrils were absent from this collar in the fetus and increased with the subject's age. A permanganate-high iron diamine sequence appeared to impart density to the microfibrillar component of elastic fibers.