奇异变形杆菌、普通变形杆菌和摩根氏变形杆菌的噬菌体分型
Bacteriophage typing of Proteus mirabilis, Proteus vulgaris, and Proteus morganii.
作者信息
Schmidt W C, Jeffries C D
出版信息
Appl Microbiol. 1974 Jan;27(1):47-53. doi: 10.1128/am.27.1.47-53.1974.
A bacteriphage typing scheme for differentiating Proteus isolated from clinical specimens was developed. Twenty-one distinct patterns of lysis were seen when 15 bacteriophages isolated on 8 Proteus mirabilis, 1 P. vulgaris, and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Seven phages isolated on 3 P. morganii were used to type 13 of 19 (68.4%) P. morganii isolates. Overall, 84.1% of the 208 isolates were lysed by at least 1 phage at routine test dilution (RTD) or 1,000 x RTD. Fifty isolates, retyped several weeks after the initial testing, showed no changes in lytic patterns. The phages retained their titers after storage at 4 C for several months. A computer analysis of the data showed that there was no relationship between the source of the isolate and bacteriophage type. This bacteriophage typing system may provide epidemiological information on strains involved in human infections.
开发了一种用于区分从临床标本中分离出的变形杆菌的噬菌体分型方案。当用从8株奇异变形杆菌、1株普通变形杆菌和1株摩根氏菌中分离出的15种噬菌体对189株（85.7%）奇异变形杆菌和普通变形杆菌分离株中的162株进行分型时，观察到21种不同的裂解模式。用从3株摩根氏菌中分离出的7种噬菌体对19株（68.4%）摩根氏菌分离株中的13株进行分型。总体而言，在常规试验稀释度（RTD）或1000×RTD下，208株分离株中有84.1%至少被1种噬菌体裂解。在初次检测几周后重新分型的50株分离株，其裂解模式没有变化。这些噬菌体在4℃保存几个月后仍保持其效价。对数据的计算机分析表明，分离株的来源与噬菌体类型之间没有关系。这种噬菌体分型系统可能为涉及人类感染的菌株提供流行病学信息。
Zotero 插件